Human Anti-Biotin Recombinant Antibody (HPAB-0109-YJ)
CAT#: HPAB-0109-YJ
This product is a recombinant human antibody that recognizes Biotin. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Tested Data
WB
Figure 1 Recombinant Humanized Anti-Biotin Antibody (HPAB-0109-YJ) in WB
Western blot analysis of HPAB-0109-YJ was performed with Biotin (His Tag).
Lane 1: Reducing antigen (0.2μg)
Lane 2: Reducing antigen (0.4μg)
Lane 3: Reducing antigen (0.8μg)
The secondary antibody: HRP Goat Anti-Human IgG (H+L)
ELISA
Figure 2 Recombinant Humanized Anti-Biotin Antibody (HPAB-0109-YJ) in ELISA
ELISA analysis of HPAB-0109-YJ was performed by coating with biotin (His Tag).
The secondary antibody: HRP Goat Anti-Human IgG (H+L)
SDS-PAGE
Figure 3 Human Anti-Biotin Recombinant Antibody (clone huM33) (HPAB-0109-YJ) in SDS-PAGE
SDS-PAGE analysis of HPAB-0109-YJ in reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
The purity of HPAB-0109-YJ was greater than 95% as determined by SDS-PAGE.
SEC-HPLC
Figure 4 Human Anti-Biotin Recombinant Antibody (clone huM33) (HPAB-0109-YJ) in HPLC
The purity of HPAB-0109-YJ was greater than 95% as determined by SEC-HPLC.
WB
Figure 5 Human Anti-Biotin Recombinant Antibody (clone huM33) (HPAB-0109-YJ) in WB
Western blot analysis of HPAB-0109-YJ was performed by loading recombinant protein with biotin labeled.
HPAB-0109-YJ incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-human IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
ELISA
Figure 6 Human Anti-Biotin Recombinant Antibody (clone huM33) (HPAB-0109-YJ) in ELISA
ELISA analysis of HPAB-0109-YJ was performed by coating with recombinant protein with biotin labeled.
The secondary antibody: HRP-goat anti-human IgG
SDS-PAGE
Figure 7 Afuco™ Human Anti-Biotin Recombinant Antibody (clone huM33) (HPAB-0109-YJ) in SDS-PAGE
SDS-PAGE analysis of Afucosylated HPAB-0109-YJ in reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
The purity of Afucosylated HPAB-0109-YJ was greater than 95% as determined by SDS-PAGE.
WB
Figure 8 Afuco™ Human Anti-Biotin Recombinant Antibody (clone huM33) (HPAB-0109-YJ) in WB
Western blot analysis of Afucosylated HPAB-0109-YJ was performed by loading a antibody with biotin conjugated.
Afucosylated HPAB-0109-YJ incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-human IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
ELISA
Figure 9 Afuco™ Human Anti-Biotin Recombinant Antibody (clone huM33) (HPAB-0109-YJ) in ELISA
ELISA analysis of Afucosylated HPAB-0109-YJ was performed by coating with a antibody with biotin conjugated. Then blocked with BSA, and incubated with Afucosylated anti-Biotin recombinant antibody (clone huM33) (HPAB-0109-YJ). The goat anti-human IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
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Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized IgG1, κ
- Specificity
- Chemical
- Species Reactivity
- Chemical
- Applications
- SPR
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Biotin
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-0109-YJ, RRID: AB_3111356)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0107-YJ | Mouse Anti-Biotin Recombinant Antibody (HPAB-0107-YJ) | SPR | Mouse IgG1, κ |
| HPAB-0108-YJ | Human Anti-Biotin Recombinant Antibody (HPAB-0108-YJ) | SPR | Chimeric (mouse/human) IgG1, κ |
| HPAB-S0187-YC | Mouse Anti-Biotin Recombinant Antibody (clone M33) | ELISA | Mouse IgG |
| 2RD-A1556-YJ | HRP-conjugated Streptavidin | ELISA, WB | |
| VS-0224-XY14 | Rabbit Anti-Biotin Recombinant Antibody (clone chiBK-1/39) | FC, IP, IHC, ChIP | Rabbit IgG, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0107-YJ-S(P) | Mouse Anti-Biotin Recombinant Antibody; scFv Fragment (HPAB-0107-YJ-S(P)) | SPR | Mouse scFv |
| HPAB-0108-YJ-S(P) | Mouse Anti-Biotin Recombinant Antibody; scFv Fragment (HPAB-0108-YJ-S(P)) | SPR | Mouse scFv |
| HPAB-0109-YJ-S(P) | Human Anti-Biotin Recombinant Antibody; scFv Fragment (HPAB-0109-YJ-S(P)) | SPR | Humanized scFv |
| HPAB-S0187-YC-S(P) | Mouse Anti-Biotin Recombinant Antibody (clone M33); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0107-YJ-F(E) | Mouse Anti-Biotin Recombinant Antibody; Fab Fragment (HPAB-0107-YJ-F(E)) | SPR | Mouse Fab |
| HPAB-0108-YJ-F(E) | Human Anti-Biotin Recombinant Antibody; Fab Fragment (HPAB-0108-YJ-F(E)) | SPR | Human Fab |
| HPAB-0109-YJ-F(E) | Human Anti-Biotin Recombinant Antibody; Fab Fragment (HPAB-0109-YJ-F(E)) | SPR | Humanized Fab |
| HPAB-S0187-YC-F(E) | Mouse Anti-Biotin Recombinant Antibody (clone M33); Fab Fragment | ELISA | Mouse Fab |
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